Detection of point mutations in genes encoding gtp binding...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2075053

2075053 9112343 PCTABS00006 The present invention provides a method for detecting whether a point mutation is present in a nucleic acid encoding a GTP bindingprotein or protein subunit. Methods are also provided for characterizing point mutations, if present. In a preferred embodiment, the method involves amplification of a nucleic acid segment followed by sequence-specific probe hybridization. The method is preferred for nucleic acids which encode a G-protein .alpha. subunit or p21 ras protein.

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