C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/37 (2006.01) B82Y 5/00 (2011.01) C12Q 1/34 (2006.01) C40B 40/10 (2006.01) C40B 70/00 (2006.01) G01N 21/65 (2006.01) G01N 33/52 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01)
Patent
CA 2679707
This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.
La présente invention concerne la détection in vitro de protéases au moyen de sondes uniques de spectroscopie laser de l'effet Raman exalté de surface (SERS) à nanocroissant conjugué à un peptide, avec au moins une sensibilité d'ordre nanomolaire. La sonde permet de détecter l'activité protéolytique dans un volume extrêmement petit et à faible concentration. Dans certains modes de réalisation, les sondes comportent un indicateur de détection d'une protéase active, l'indicateur étant constitué d'un nanocroissant fixé à un peptide et ledit peptide comportant un site de reconnaissance de la protéase ainsi qu'un marqueur Raman fixé à ce peptide.
Chen Fanqing Frank
Ellman Jonathan A.
Lee Luke P.
Liu Gang L.
Cassan Maclean
The Regents Of The University Of California
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