Detection of ras mutations

Details Detection of ras mutations Detection of ras mutations

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01) G01N 27/26 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2424584

Mutations in K-ras, N-ras, and H-ras were determined using target specific primers and probes in REMS-PCR methods, nested PCR methods employing a restriction endonuclease, and REMS-PCR methods using molecular beacons.

On a déterminé des mutations de K-ras, N-ras et H-ras au moyen d'amorces et de sondes spécifiques pour une cible au moyen de procédés REMS-PCR, de procédés PCR imbriqués mettant en application une endonucléase de restriction, ainsi que de procédés REMS-PCR utilisant des phares moléculaires.

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