Detection of rpob sequences of mycobacterium tuberculosis

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2443534

A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific primer sequences is disclosed. Compositions for detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.

L'invention concerne un procédé de détection de séquences de rpoB de Mycobacterium tuberculosis présentes dans un échantillon biologique, ce procédé comprenant l'opération consistant à amplifier in vitro la séquence de rpoB de M. tuberculosis, dans un mélange d'amplification d'acide nucléique qui comprend des séquences amorces spécifiques. L'invention concerne également des compositions utilisées pour la détection in vitro de séquences de rpoB de M. tuberculosis dans un échantillon.

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