Detection of saliva proteins modulated secondary to ductal...

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G01N 33/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2700125

A method of differentiating among the presence of ductal carcinoma in situ in the breast, benign fibroadenoma of the breast, and non-cancerous breast tissue in a subject is disclosed. The method comprises: measuring the concentration of at least one protein biomarker selected from a group of forty-nine differentially expressed proteins in the saliva of persons with DCIS, or benign fibroadenoma, or in persons who are cancer-free. The resulting test data is compared to a reference panel. From the comparison the presence in the subject of either ductal carcinoma in situ of the breast, or benign fibroadenoma of the breast is determined.

La présente invention concerne une méthode qui permet de différencier chez un sujet la présence d'un carcinome canalaire in situ (CCIS) du sein, d'un fibroadénome bénin du sein et de tissus mammaires non cancéreux. La méthode consiste à mesurer la concentration d'au moins un biomarqueur de protéine sélectionné dans un groupe de quarante-neuf protéines exprimées de manière différentielle dans la salive de personnes atteintes de CCIS ou de fibroadénome bénin ou encore chez des personnes qui ne sont pas atteintes d'un cancer. Les données de test obtenues sont comparées à un groupe de référence. Sur la base de la comparaison, on détermine la présence chez le sujet d'un carcinome canalaire in situ du sein ou d'un fibroadénome bénin du sein.

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