Detection of salmonella by real-time multiplex pcr

Details Detection of salmonella by real-time multiplex pcr Detection of salmonella by real-time multiplex pcr

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2661484

The invention relates to the detection of Salmonella by nucleic acid amplification. The invention provides primer and probe oligonucleotides that can be used in multiplex to detect Salmonella in real-time amplification. The oligonucleotides of the invention detect all group I serovars, and have an increased Salmonella detection range: they enable to cover the seven Salmonella groups. They also have an increased sensitivity, without loss in specificity.

L'invention concerne la détection de salmonelles par amplification d'acides nucléiques. L'invention concerne également des oligonucléotides d'amorce et de sonde pouvant être employés en multiplexage afin de détecter des salmonelles dans l'amplification en temps réel. Les oligonucléotides selon l'invention détectent tous les sérotypes du groupe (I) et présentent une plus grande palette de détection de salmonelles: ils permettent de couvrir les sept groupes de salmonelles. Lesdits oligonucléotides présentent également une plus grande sensibilité, sans perte de spécificité.

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