Detection of shed cd31, diagnosis of atherothrombosis and...

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G01N 33/68 (2006.01) C07K 16/28 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2729584

The present invention stems from the finding that the extracellular domain of CD31 proteins present on blood leukocytes is shed and released in the circulation as a soluble form of CD31. A method for detecting shed CD31 is further disclosed. The invention therefore relates to a method for detecting a shed ectodomain of a transmembrane protein such as CD31 and to the use of such a method as a diagnostic tool. The invention further provides methods for determining whether a candidate protein is part of a molecular complex.

La présente invention concerne la découverte selon laquelle le domaine extracellulaire des protéines CD31 présentes sur les leucocytes sanguins se détache et est libéré dans la circulation sous la forme dune forme soluble de CD31. Linvention concerne également un procédé de détection des CD31 détachées. Linvention concerne par conséquent un procédé de détection dun ectodomaine détaché dune protéine transmembranaire telle que CD31, et lutilisation dun tel procédé en tant quoutil de diagnostic. Linvention concerne également des procédés de détermination de lappartenance dune protéine candidate à un complexe moléculaire.

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