Detection of targets with green fluorescent protein and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) A01N 47/38 (2006.01) C07D 307/16 (2006.01) C07D 309/14 (2006.01) C07D 309/28 (2006.01) C07D 405/12 (2006.01) C07D 405/14 (2006.01) C07D 409/14 (2006.01) C07D 413/14 (2006.01) C07H 19/04 (2006.01) C07H 21/04 (2006.01) C07K 1/00 (2006.01) C12N 9/02 (2006.01) C12P 21/04 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2283094

Green fluorescent protein or fluorescent variants thereof are combined with ligands for use as labeled markers in detection of targets. Suitable ligands include nucleic acid probes, antibodies, hapten conjugates, biotin, avidin and streptavidin. Techniques such as fluorescent microscopy are used to visualize the labeled marker.

On combine une protéine fluorescente verte ou ses variantes fluorescentes avec des ligands afin de les utiliser en tant que marqueurs fluorescents dans la détection de cibles. Les ligands appropriés comprennent des sondes d'acide nucléique, des anticorps, des conjugués d'haptènes, biotine, avidine et streptavidine. On met en application des techniques, telles que la microscopie fluorescente, afin visualiser le marqueur.

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