Detection of the presence of biological activity utilizing...

Details Detection of the presence of biological activity utilizing... Detection of the presence of biological activity utilizing...

G - Physics

01

N

150/16, 73/59

G01N 21/59 (2006.01) C12M 1/34 (2006.01)

Patent

CA 1204606

P-535 DETECTION OF THE PRESENCE OF BIOLOGICAL ACTIVITY UTILIZING INFRARED ANALYSIS ABSTRACT OF THE DISCLOSURE A method and apparatus for detecting the presence of biological activity in a sample of material. In the method, a sample of the material is placed in a closed container with a growth medium which includes a carbon source which may be metabo- lized to produce carbon dioxide. The medium with the sample therein is exposed to conditions conducive to the occurrence of normal metabolic process for a period of time sufficient to cause production of CO2 by the metabolism of said carbon source. Thereafter, the presence of CO2 in the gaseous atmosphere above the medium is detected by measuring the infrared absorbance of the gaseous atmosphere with the container by passing an infrared beam through the container and the gaseous atmosphere and detecting the infrared absorbance of the gaseous atmosphere.

435797

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