Detection of toxigenic strains of clostridium difficile

Details Detection of toxigenic strains of clostridium difficile Detection of toxigenic strains of clostridium difficile

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C07K 14/33 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2698232

Primers and probes for detection of toxin-producing (toxigenic) strains of Clostridium difficile, and to methods of detecting toxigenic strains using these primers and probes. Toxigenic strains of C. difficile are detected by nucleic acid-based amplification methods using particular primers and probes that bind to the toxin B (TcdB) gene. These primers and probes are used to amplify C. difficile nucleic acids in clinical samples to determine the presence of these toxigenic strains.

Amorces et sondes pour la détection de souches génératrices de toxines (toxigènes) de Clostridium difficile, et méthodes de détection de tels souches au moyen de ces amorces et sondes. Pour la détection de souches de Clostridium difficile, on utilise des méthodes d'amplification à base d'acides nucléiques au moyen d'amorces et de sondes particulières se liant à des gènes de toxine B (TcdB). Ces amorces set sondes servent à amplifier les acides nucléiques de C. difficile dans des échantillons cliniques pour déterminer la présence de ces souches toxigènes.

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