Detection of truncation mutations by mass spectrometry

Details Detection of truncation mutations by mass spectrometry Detection of truncation mutations by mass spectrometry

G - Physics

01

N

G01N 33/53 (2006.01) C12P 21/06 (2006.01) G01N 33/00 (2006.01)

Patent

CA 2575519

This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.

La présente invention se rapporte à la détection et à l'analyse par spectrométrie de masse de protéines à l'état naissant, et en particulier de protéines tronquées, traduites dans des systèmes de traduction cellulaires ou acellulaires. Des épitopes N-terminaux et C-terminaux introduits dans lesdites protéines à l'état naissant permettent un isolement rapide et efficace, ainsi qu'une différence de masse.

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