Detection of wheat and barley fungal pathogens using the...

Details Detection of wheat and barley fungal pathogens using the... Detection of wheat and barley fungal pathogens using the...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2319132

Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for species and strains of wheat fungal pathogens, including Pyrenophora tritici-repentis and Pyrenophora teres. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.

L'invention concerne des séquences d'ADN du bras espaceur interne transcrit (ITS) de la région génique de l'ARN ribosomique, pour différentes espèces et souches d'agents pathogènes fongiques du blé, notamment Pyrenophora tritici-repentis et Pyrenophora teres. Les amorces spécifiques tirées de ces séquences se sont avérées utiles pour identifier des isolats fongiques, grâce à des techniques basées sur une réaction en chaîne de la polymérase (PCR).

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