Detection or isolation of target molecules using a...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 3/00 (2006.01) C12M 1/26 (2006.01) C12Q 1/00 (2006.01) C12Q 1/24 (2006.01) G01N 33/53 (2006.01) G01N 33/567 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2658336

A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.

L'invention concerne un appareil à micro-écoulement pour séparer ou isoler des cellules à partir d'un fluide corporel ou d'un autre échantillon de liquide, lequel appareil utilise un chemin d'écoulement dans lequel un écoulement en ligne droite est interrompu par un motif de montants transversaux. Les montants sont espacés sur la largeur d'une zone de collecte située sur le chemin d'écoulement et s'étendant entre la surface supérieure et la surface inférieure de celle-ci. Les montants présentent des surfaces rectilignes, des coupes transversales arquées et sont disposés au hasard de façon à interrompre un écoulement direct. Des agents de séquestration, tels que des anticorps, sont fixés à toutes les surfaces dans la zone de collecte par l'intermédiaire d'un revêtement hydrophile, de préférence un hydrogel contenant des fractions isocyanates ou un PEG ou une polyglycine de longueur substantielle, et sont hautement efficaces pour capturer des cellules ou autres biomolécules ciblées résultant d'une telle interruption d'écoulement direct.

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