G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/543 (2006.01) G01N 33/564 (2006.01) G01N 33/571 (2006.01) G01N 33/68 (2006.01) G01N 33/92 (2006.01)
Patent
CA 2021946
ABSTRACT This method can determinate and detect an antibody in a biological fluid sample against a specific antigen and its immunoglobrin class. This method comprises the step of: -----providing a first reagent of insoluble substrate particles carrying the antigen on the surface thereof and a second reagent of insoluble substrate particles carrying on the surface thereof a substance paticulary reactive to a specific immunogloblin class, the latter reagent containing a magnetic material; -----reacting the sample with both of the reagents in a reaction system; -----agglomerating the antigen-carrying particles together with the magnetic material-containing particles through the antibody; -----separating both of the unreacted magnetic material- containg particles and the resultant agglomerate by applying a magnetic field to the reaction system; and -----visually or spectrophotometrically estimating an amount of the antigen-carrying particles which remains unreacted in the reaction system.
Ito Michio
Kohno Hideki
Ogura Minoru
Goudreau Gage Dubuc
Mitsubishi Chemical Corporation
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