Determination and identification of active compounds in a...

G - Physics – 01 – N

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G01N 33/53 (2006.01) C07H 21/00 (2006.01) C07K 1/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2185918

Libraries of compounds such as nucleic acids or peptides are contacted with a target molecule and libraries that have at least one compound that binds with at least a minimum activity are determined by a reiterative process in which a change in the rate of recorvery (elimination) of compounds that bind to the target indicates that the library contains such a compound. The procedure may also be used to determine indirectly the sequence of such compound by employing sublibraries, each of which has a known entity at a known position of the compound.

Des bibliothèques de composés, tels que les acides nucléiques ou les peptides, sont mises en contact avec une molécule cible. Des bibliothèques comportant au moins un composant se liant avec au moins une activité minimale, subissent un processus répétitif de détermination au cours duquel la modification du taux de récupération (d'élimination) des composés se liant avec la cible signale que la bibliothèque contient un tel composé. Le procédé permet également de déterminer indirectement la séquence d'un tel composé grâce à des sous-bibliothèques comportant chacune une entité connue en une position connue du composé.

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