Determination method, use and components

Details Determination method, use and components Determination method, use and components

G - Physics

01

N

167/37

G01N 33/53 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01) G01N 33/74 (2006.01) G01N 33/94 (2006.01)

Patent

CA 1340488

A method as described for determining a hapten which method comprises (i) contacting the hapten with a binding partner of the hapten, whereby the hapten becomes bound to some of the binding partner, (ii) contacting the unbound binding partner with a secondary binding partner therefore, (iii) contacting the binding partner with an antibody which binds the binding partner which has bound thereto the hapten but which does not bind the binding partner which has bound thereto its secondary binding partner; and (iv) determining the amount of antibody bound to the binding partner. Kits for use in the method are also described.

614043

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