Determination of a specific immunoglobulin using multiple...

Details Determination of a specific immunoglobulin using multiple... Determination of a specific immunoglobulin using multiple...

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/564 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2195753

The invention concerns a process for the immunological determination of a specific antibody in a fluid sample. The process involves incubating the fluid sample in the presence of a solid phase with two antigens directed against the antibody whose presence is to be determined; the first antigen carries at least one marker group, while the second is either (a) bonded to the solid phase or (b) present in a form in which it can bond with the solid phase, and betrays the presence of the antibody being sought by showing the presence of the marker group in the solid phase and/or in the fluid phase. The proposed process is characterised by the fact that at least one of the two antigens has several epitopic regions which react with the antibody to be determined.

L'invention concerne un procédé pour le dosage immunologique d'un anticorps spécifique dans un échantillon de liquide. Ce procédé consiste à incuber l'échantillon de liquide en présence d'une phase solide avec deux antigènes dirigés contre l'anticorps à doser; le premier antigène porte au moins un groupe marqueur, tandis que le second est soit (a) lié à la phase solide ou (b) présent sous une forme dans laquelle il peut se lier à la phase solide, et décèle l'anticorps à doser par recherche du groupe marqueur dans la phase solide et/ou dans la phase liquide. Le procédé décrit est caractérisé en ce qu'au moins un des deux antigènes comporte plusieurs régions d'épitope qui réagissent avec l'anticorps à doser.

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