Determination of aminotransferases

Details Determination of aminotransferases Determination of aminotransferases

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/26 (2006.01) C12Q 1/32 (2006.01) C12Q 1/52 (2006.01)

Patent

CA 2011981

ABSTRACT OF THE DISCLOSURE A method and reagent system for determining the amount of an aminotransferase present in a biological test sample. The aminotransferase is determined by contacting a test sample containing an aminotransferase with its corresponding amino acid, a ketoacid, a dehydrogenase or a reductase, and a reduced form of thionicotinamide adenine dinculeotide or an analog thereof to form a liquid mixture thereof. The visible absorbance of the liquid mixture is measured and correlated to the amount of aminotransferase present in the test sample. The method and reagent system of the present invention are particularly useful for determining alanine aminotransferase and aspartate aminotransferase.

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