Determination of an analyte in a liquid medium

G - Physics – 01 – N

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G01N 33/543 (2006.01) C12M 1/40 (2006.01) C12Q 1/00 (2006.01) C12Q 1/26 (2006.01) C12Q 1/54 (2006.01) G01N 27/416 (2006.01) G01N 33/553 (2006.01)

Patent

CA 2244214

Electrochemical determination of the presence, and optionally concentration of an analyte utilizes an electrode having immobilized thereon one member of a recognition pair, the recognition pair consisting of said one member and of said analyte. In the presence of the analyte in the medium pair complexes, which are complexes between the analyte and the immobilized member are formed. The electrode forms part of an electrochemical system which includes also redox molecules comprising at least one first redox molecule and at least one second redox molecule, the former transferring electrons between the electrode and the latter. Upon formation of a pair complex the electron transfer is inhibited and the change in the electrical response then indicates the presence of said analyte in the medium.

La détermination électrochimique de la présence et éventuellement de la concentration d'un analyte fait appel à une électrode sur laquelle est immobilisé un élément d'un couple de reconnaissance, ce dernier étant constitué dudit élément et dudit analyte. En présence de l'analyte dans le milieu se forment des complexes de couples qui sont des complexes entre l'analyte et l'élément immobilisé. L'électrode fait partie d'un système électrochimique comportant également des molécules redox comprenant au moins une première molécule redox et au moins une deuxième molécule redox, ladite première molécule transférant des électrons entre l'électrode et ladite deuxième molécule. Lors de la formation d'un complexe de couples, le transfert d'électrons est inhibé et la variation de la réponse électrique indique alors la présence dudit analyte dans le milieu.

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