C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
167/139, 167/45
C12Q 1/28 (2006.01) A61K 39/395 (2006.01) G01N 33/574 (2006.01)
Patent
CA 1185526
Abstract A solid phase-sandwich method for the rapid determination of carcinoembryonic antigen (CEA) is described. According to this method, the sample to be investigated is incubated with a first CEA antibody, which is bound to or becomes bound to a water-insoluble carrier, and a second CEA anti- body, to which peroxidase is bound directly or via a biotin/ avidin bridge. The immunological reaction can be carried out in one or two steps. By the presence of 0.4 1.0 mol/l of phosphate ions, 0.2-0.4 mol/l of sulphate ions or 0.2-0.4 mol/l of tartrate ions in the immunological reaction or in the second step thereof (insofar as it is carried out in two steps) the incubation duration is shortened considerably. After the immunological reaction, the phases are separated, whereupon the peroxidase activity is measured either in the solid or in the liquid phase*as the amount of CEA present in the sample.
406742
Brodbeck Hans
Gallati Harald
Gowling Lafleur Henderson Llp
Hoffmann-La Roche Limited
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