Determination of cells in blood

Details Determination of cells in blood Determination of cells in blood

G - Physics

01

N

150/13

G01N 31/02 (2006.01) A61B 5/145 (2006.01) G01N 31/22 (2006.01) G01N 33/48 (2006.01) G01N 33/487 (2006.01)

Patent

CA 1077813

ABSTRACT OF THE INVENTION A method for determining a volume of red cells in a blood sample. The method includes the steps of flowing along a conduit a predetermined volume of the sample, introducing an agglutinating reagent and a predetermined volume of a dye into the conduit to mix with the sample and agglutinate the red cells, the dye being of known concentration on the introduction thereof, decanting the agglutinated red cells from the conduit, and colorimetrically measuring the concentration of the dye in the conduit, the agglutinating reagent being characterized by a relatively high positive electrical charge and the dye being water soluble and characterized by not penetrating said cells and not forming a precipitate with the agglutinating reagent or with the soluble components of the sample and not lysing the cells or altering the volume of the cells.

292875

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