Determination of glycated hemoglobin by fluorescence quenching

G - Physics – 01 – N

Patent

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G01N 33/72 (2006.01) C07K 1/22 (2006.01) G01N 21/64 (2006.01) G01N 33/52 (2006.01) C07K 14/805 (2006.01)

Patent

CA 2102417

The present invention relates to the measurement of glycated hemoglobin by fluorescence quenching. The present invention uniquely involves performing two sequential fluorescent quenching measurements: one measurement of the fluorescent quenching due to total hemoglobin in the sample and a second measurement of the fluorescent quenching due to glycated hemog-lobin present in the sample after the non-glycated hemoglobin is removed. Glycated hemoglobin and non-glycated hemoglobin can be separated by a variety of methods as described herein, including ion capture and solid phase separations.

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