Determination of glycosilated haemoglobin

G - Physics – 01 – N

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150/11, 150/3

G01N 33/72 (2006.01) G01N 33/66 (2006.01)

Patent

CA 1199257

ABSTRACT Process and reagent for the determination of glyco- silated haemoglobin The present invention provides a process for the determination of glycosilated haemoglobin in a blood sample in which glycosilated and non-glycosilated haemoglobin is first liberated from the erythrocytes by chemical or physical treatment, the haemoglobin is, if desired, converted into methaemoglobin, thereafter a differentiation of the glycosilated and non- glycosilated haemoglobin is carried out and subsequently the glycosilated and/or the non-glycosilated portion of the haemoglobin is determined, wherein the different- iation of the glycosilated and non-glycosilated haemo- globin is carried out with the help of haptoglobin. The present invention also provides a reagent for the determination of glycosilated haemoglobin in a blood sample, as well as a reagent for the different- iation of glycosilated and non-glycosilated haemoglobin.

413594

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