Determination of high molecular weight analytes

Details Determination of high molecular weight analytes Determination of high molecular weight analytes

C - Chemistry, Metallurgy

12

Q

C12Q 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2060377

MCRO-022/00 US DETERMINATION OF HIGH MOLECULAR WEIGHT ANALYTES ABSTRACT A method for detecting the presence of a high- molecular-weight analyte, which uses a variation on complementation assays, comprising (1) providing an enzyme donor (ED)complex comprising ED coupled to an analyte-specific binding molecules wherein the ED complex retains measurable complementation activity such that active .beta.-galactosodase formed in the presence of an enzyme acceptor (EA); (2) contacting the ED complex and EA in the presence of a sample suspected of containing a high-molecular-weight analyte that reacts specifically with the analyte specific binding molecule; and (3) relating the presence of the analyte in the sample to the formation of active .beta.-galactosidase enzyme. 20216638 26

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