Determination of lecithin

Details Determination of lecithin Determination of lecithin

C - Chemistry, Metallurgy

12

Q

150/3.3, 150/15.

C12Q 1/42 (2006.01) C12Q 1/34 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 1184101

ABSTRACT OF THE DISCLOSURE The present invention provides a process and reagent for the determination of lecithin in a sample, and for the splitting of lecithin; in particular lecithin is split with, for example, phospholipase-C and alkaline phosphatase to give choline, this is reacted in the presence of adenosine triphosphate with the help of choline kinase again to give phosphoryl- choline and the thereby formed adenosine diphosphate is measured in known manner; in accordance with the invention the splitting of the lecithin is carried out in the presence of a borate/borax buffer and of a detergent, particularly a non-ionic detergent, based on a condensation product of polyethyleneglycol with isooctylphenol.

407494

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