C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/60 (2006.01) C12M 1/40 (2006.01) G01N 33/49 (2006.01) G01N 33/92 (2006.01) G01N 35/08 (2006.01)
Patent
CA 2143425
A continuous flow analysis system measures the cholesterol distribution among different lipoprotein classes in a blood sample. A blood plasma sample is separated into different lipoprotein classes by single vertical spin density gradient ultracentri- fugation. The sample is then introduced in a continuous succession into a continuous reagent stream which flows through a detec- tor. A signal of the reaction, such as the absorbance of the reaction product, is continuously monitored as the reaction mixture flows through the detector. The detector measures a transient signal thus allowing a relatively short reaction period. The absorb- ance data is digitized and analyzed with the help of a computer to generate a cholesterol profile indicative of the cholesterol con- centration in each lipoprotein class.
Kulkarni Krishnaji R.
Segrest Jere P.
Mcfadden Fincham
Uab Research Foundation
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