G - Physics – 01 – N
Patent
G - Physics
01
N
167/37
G01N 33/53 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2011099
INVENTORS: Robert A. Levine Stephen C. Wardlaw TITLE: "DETERMINATION OF LYMPHOCYTE REACTIVITY TO SPECIFIC ANTIGENS IN BLOOD" DOCKET NO.: H-1152 ABSTRACT OF THE DISCLOSURE A patient's blood sample is incubated with an antigen and tested for lymphocyte response, ie. an activation of lymphocytes and/or a conversion of lymphocytes to lymphoblasts, which indicates prior exposure of the patient to the antigen. A positive response indicates the presence of prior exposure to prior diseases or clinical conditions such as parasitic diseases, tuberculosis, salmonellosis, gonorrhea, fungal infections, rickettsial infections, Lyme disease or allergens. Whole blood from the patient is incubated with the antigen of the disease or condition for which the patient is being tested. After a suitable time, a fluorescent dye or colorant which has an affinity for a discriminant characteristic of the activated lymphocytes or lymphoblasts, such as: intracellular calcium; surface activation antigens such as transferrin receptor; HLA-Dr; Leu-23; and the like. The incubated blood is then drawn into a transparent tube containing a float which concentrates the buffy coat constituent layers upon centrifugation of the blood sample. The concentrated lymphocyte layer is then examined for fluorescence or coloration which is indicative of the presence of the activated lymphocytes or lymphoblasts, and their concentration. The fluorescence or coloration can be qualified and/or quantified by a reader instrument.
Levine Robert A.
Wardlaw Stephen C.
Levine Robert A.
Riches Mckenzie & Herbert Llp
Wardlaw Stephen C.
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