Determination of polyunsaturated fat levels in body fluids

Details Determination of polyunsaturated fat levels in body fluids Determination of polyunsaturated fat levels in body fluids

G - Physics

01

N

150/15.1, 150/3.

G01N 27/26 (2006.01) C12Q 1/00 (2006.01) C12Q 1/26 (2006.01) G01N 33/487 (2006.01)

Patent

CA 1095388

ABSTRACT OF THE DISCLOSURE. A method of determining the polyunsaturated fatty acid levels in a body fluid such as serum or plasma includes the steps of converting the polyunsaturated fatty acid content of a sample of body fluid into free acid or salt form, taking a predetermined volume of this body fluid, oxidising the polyunsaturated fatty acids or salts in the volume of body fluid with molecular oxygen in the presence of excess of an oxygenase enzyme which is specific for polyunsaturated fatty acids which contain a cis,cis-1,4-pentadiene system in a suitable buffer, and measuring the amount of oxygen consumed by the volume of body fluid by means of an oxygen electrode. A reagent for use in the above method comprises a borate buffer of molarity 0,1 to 2 molar and pH 7 to 10 and contains an oxygenase enzyme which is specific for polyunsaturated fatty acids containing a cis,cis-1,4-pentadiene system.

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