Determination of protein

Details Determination of protein Determination of protein

G - Physics

01

N

150/14

G01N 33/68 (2006.01) C09K 3/00 (2006.01)

Patent

CA 1129322

Abstract An aqueous reagent system for the quantita- tive determination of protein is disclosed. The sys- tem contains Coomassie Brilliant Blue G-250 dye, a monobasic strong acid having a pKa of less than 3 (e.g. HC1), a polybasic phosphonic acid (e.g. NTP), and a water soluble, nonionic surfactant characterized as being a block copolymer containing terminal polyethylene oxide segments separated by a polypropylene oxide segment. Preferably the sys- tem also contains a small amount of an alcohol such as methanol or ethanol.

341044

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