Determination of the dna methylation level

Details Determination of the dna methylation level Determination of the dna methylation level

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2749196

The present invention provides oligonucleotides and processes for determining the normalized methylation level of DNA, and for determining the relative methyla-tion level of DNA between at least two samples. The invention makes use of the random distribution of transposons in the genome. The disclosed oligonucleotides and processes are of importance, in particular, for clinical diagnostics.

La présente invention concerne des oligonucléotides et un procédé pour déterminer le degré de méthylation normé de l'ADN, ainsi que le degré de méthylation relatif de l'ADN entre au moins deux échantillons. Selon l'invention, on se sert de la répartition aléatoire de transposons dans le génome. Les oligonucléotides et le procédé de l'invention ont une utilité importante notamment pour le diagnostic clinique.

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