G - Physics – 01 – N
Patent
G - Physics
01
N
150/11
G01N 23/00 (2006.01)
Patent
CA 1092956
ABSTRACT OF THE DISCLOSURE A process and test kit for the quantitative determination of the individual and total unsaturated Vitamin B-12 binding capacities of the three transcobalamins TC-I, TC-II and TC-III in serum. The process comprises incubating a serum sample with a solution of 57Co Vitamin B-12, contacting the resulting mixture with a TC-II adsorbent and with a DEAE-cellulose adsorbent for TC-I and TC-III, measuring the total radioactivity adsorbed to said adsorbents, selectively desorbing TC-III from the DEAE- cellulose adsorbent by washing with a monopotassium phosphate solution of about 0.05 M and at a pH of about 4.6, measuring the individual radioactivity adsorbed to each of said adsorbents, and calculating the radioactivity that had been washed from the DEAE- cellulose adsorbent by said phosphate solution. The resulting four radioactivity values are functions of the total, TC-II, TC-I, and TC-III unsaturated Vitamin B-12 binding capacities, respectively, of the serum tested. The test kit comprises the essential elements for performing the present process.
278950
Grossowicz Nathan
Rachmilewitz Bracha
Selhub Jacob
Osler Hoskin & Harcourt Llp
Yissum Research Development Company Of The Hebrew University Of
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