B - Operations – Transporting – 01 – L
Patent
B - Operations, Transporting
01
L
B01L 3/00 (2006.01) C12M 1/34 (2006.01) C12M 3/00 (2006.01) C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) C12Q 1/06 (2006.01) C12Q 1/22 (2006.01) G01N 15/06 (2006.01) G01N 33/00 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/544 (2006.01) G01N 33/551 (2006.01) G01N 33/553 (2006.01) G01N 33/567 (2006.01)
Patent
CA 2598937
The present invention relates to methods for the detection of microorganisms. In one embodiment, the present invention provides methods for detecting live microorganisms in a culture by capturing and culting the microorganisms on paratropic-coated paramagnetic beads. This technique is useful for any application in which it is necessary to monitor the biological contamination level, for example drinking water, recreational waters, food processing waters and medical laboratories.In one embodiment, , the method for determining the concentration of viable microorganisms in a sample according to the invention further comprises an inducer reagent, wherein the inducer reagent includes an inducer compound that induces the activity of an enzyme usinque to the microorganism of interest.
La présente invention concerne des procédés de détection de micro-organismes. Dans un mode de réalisation, l'invention est un procédé de détection de micro-organismes vivants dans une culture par capture et mise en culture des micro-organismes sur des billes paramagnétiques à revêtement paratrophe. Cette technique convient pour toute application demandant une surveillance du niveau de contamination biologique, (eaux de boisson, eaux de baignade, eaux de cuisine, et laboratoires médicaux). Dans un mode de réalisation, le procédé, qui permet d'évaluer la concentration en micro-organismes viables dans un échantillon, comporte un réactant d'induction incluant un composé inducteur induisant l'activité dans un enzyme unique pour le micro-organisme étudié.
Boyaci Ismail Hakki
Halsall Hallen Brian
Heineman William Richard
Seliskar Carl James
Macpherson Leslie & Tyerman Llp
University Of Cincinnati
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