Determining the transforming capability of agents

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12Q 1/00 (2006.01) G01N 33/50 (2006.01) G01N 33/574 (2006.01) A61K 38/16 (2006.01)

Patent

CA 2301401

The invention relates to activation of apoptin-induced apoptosis by different cell-transforming agents in normal and/or cancer-prone cells. Apoptin or also called VP3 is a viral protein derived from the Chicken anemia virus. Also the inven-tion relates to preventive anti-tumor ther-apies of normal and/or cancer-prone cells. Treatment of normal and/or cancer-prone cells with tumor-inducing agents will ac-tivate apoptin-induced apoptosis, resulting in the elimination of potential tumor cells. Also the invention relates to diagnosis of cancer agents. Agents with tumor activity can be examined by expressing them in nor-mal cells and analyzing their capability of enabling apoptin-induced apoptosis.

Cette invention a trait à l'activation de l'apoptose déclenchée par l'apoptine par différents agents ayant une action transformante à l'égard de la cellule et ce, s'agissant de cellules normales ou de cellules sujettes au cancer. L'apoptine, également appelée VP3 est une protéine virale dérivée du virus de l'anémie infectieuse du poulet. L'invention concerne également des traitements préventifs antitumoraux s'adressant à des cellules normales ou à des cellules sujettes au cancer. Le traitement de ces cellules au moyen d'agents déclenchant une tumeur a pour effet d'activer l'apoptose déclenchée par l'apoptine, ce qui entraîne l'élimination de cellules tumorales potentielles. L'invention porte, de surcroît, sur des agents diagnostiques du cancer. Il est possible, dans le cadre de cette invention, d'examiner des agents faisant montre d'une activité tumorale en les exprimant dans des cellules normales et en analysant leur capacité à stimuler l'apoptose déclenchée par l'apoptine.

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