Deuteriated substances

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/20 (2006.01) C07K 14/245 (2006.01) C12N 1/36 (2006.01) C12P 1/00 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2230051

The present invention provides a method for producing a substantially deuteriated substance comprising the steps of growing on a deuteriated medium an organism producing the substance, wherein the organism has been adapted to deuteriated medium such that on a deuteriated medium it produces approximately the same quantity of the deuteriated substance as the non-adapted organism produces of the non-deuteriated substance on a non-deuteriated medium. Also provided are substances produced according to the method of the present invention, MRE600 for use in a method of production of a substantially deuteriated substance, and substantially deuteriated EFTu having a substantially greater half-life in the presence of chymotrypsin in protiated media than protiated EFTu.

La présente invention concerne un procédé de production d'une substance pratiquement deutérée, lequel comprend les étapes consistant à faire croître sur un milieu deutéré un organisme produisant ladite substance, cet organisme étant adapté à un milieu deutéré de manière à produire sur celui-ci approximativement la même quantité de substance deutérée qu'un organisme non approprié produit de substance non deutérée sur un milieu non deutéré. On décrit également des substances produites selon le procédé de l'invention, l'organisme MRE600 utile dans un procédé de production d'une substance pratiquement deutérée, et le facteur d'élongation T¿u? (EFT¿u?) pratiquement deutéré, possédant une demi-vie sensiblement supérieure, en présence de chymotrypsine et dans des milieux marqués au protium, à celle du facteur d'élongation T¿u? marqué au protium.

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