Development of an in vivo functional assay for proteases

Details Development of an in vivo functional assay for proteases Development of an in vivo functional assay for proteases

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C12N 15/87 (2006.01) C12Q 1/02 (2006.01) C12Q 1/37 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2432676

There is provided a method of detecting protease activity in an intracellular region of a cell. The method comprises: obtaining a reporter protein having a site susceptible to cleavage by the protease of interest in the intracellular region, introducing the reporter protein into the intracellular region, and assaying the effect on reporter activity observed following its entry into the intracellular region. Also provided are protein and nucleotide sequences useful in carrying out the method. Of particular interest are protein and nucleotide sequences relating to mutant forms of Green Fluorescent Protein that are useful as a reporter protein in the intracellular protease assay.

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