Development of dna probes and immunological reagents of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/12 (2006.01) C07K 14/82 (2006.01) C07K 16/30 (2006.01) C07K 16/32 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2094378

2094378 9208131 PCTABS00013 The present invention provides a general method for identifying genes and producing immunological reagents which encode cell surface antigens of human origin. Specifically, the present invention provides a method for preparing a hybridoma cell line which produces an antibody which specifically recognizes and binds to a cell surface antigen associated with a neoplastic, human cell. This invention also provides a method for preparing a monoclonal and a polyclonal antibody which specifically recognizes and binds to a cell surface antigen associated with a neoplastic, human cell. Using the antibodies produced, this invention further provides a method for diagnosing in a subject a neoplastic condition, a method for treating a neoplastic condition, and a method for imaging a neoplastic, human cell. This invention also provides a method for preparing DNA encoding a cell surface antigen associated with a neoplastic, human cell, DNA probes hybridized with the DNA prepared therein, as well as a method for diagnosing a neoplastic condition using the DNA probes.

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