Development of mammalian genome modification technique using...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) A01K 67/027 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2546848

To comprehensively modify genome, it is intended to develop a transposition system of the copy and paste type which has an improved efficiency. This object has been achieved by the finding that an LTR retrotransposon is partly usable in a transposition system. Namely, a technique of efficiently transferring a foreign gene into a cell by using a transposon. More specifically speaking, a complete IPA element and a functional promoter sequence are found out. It is clarified that, without a combination of them, a retrotransposon cannot exert its function.

L'invention concerne la mise au point d'un système de transposition de type copier-coller, destiné à modifier entièrement un génome, et d'une efficacité améliorée. On a, en effet, découvert qu'un rétrotransposon LTR peut être partiellement utilisé dans un système de transposition. L'invention concerne ainsi une technique efficace de transfert d'un gène étranger dans une cellule à l'aide d'un transposon. L'invention concerne, plus spécifiquement, un élément IPA complet et une séquence promoteur fonctionnelle. Il est prouvé que le rétrotransposon ne peut pas exercer sa fonction sans une combinaison des deux.

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