Device and installation of thin film chromatography, with...

G - Physics – 01 – N

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73/93, 182/2.1

G01N 30/94 (2006.01) B01D 15/08 (2006.01)

Patent

CA 2035025

Improvements have been made to the developing step in thin layer chromatography, both on a developing device for carrying out the improvement, and in apparatus containing the device. The improvements are on a developing method in a thin-layer chromatography developing procedure in which a chromatographic plate to be developed is brought into contact with a material which is impregnated with a developing reagent. The improvement includes pressing, in a substantially-uniform manner, a porous polymer containing the developing reagent, against the thin-layer chromatography plate to be developed. The porous polymer possesses sufficient absorbing power completely to impregnate the chromatographic plate without immersing it during the contact under pressure, and a capacity for reabsorbing excess reagent when the porous polymer is withdrawn from the plate. The developing device for the thin layer chromatography comprises a porous polymer which is resistant to conventional chromatography developing reagents and which has sufficient absorbing power completely to impregnate a chromatographic plate without immersing it at the time of contact under pressure and to reabsorb excess reagent when the porous material is withdrawn from the chromatographic plate. The device includes a manipulation support which is inert with respect to the developing reagents, and which has sufficient rigidity to allow an appropriate pressure to be applied to the thin-layer chromatographic plate and on which the porous material is mounted or fixed.

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