Device and method for detecting antibiotic inactivating enzymes

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/18 (2006.01)

Patent

CA 2493870

The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a permeabilizing agent for the microorganism present in a non-growth inhibiting microorganism-permeabilizing effective amount.

L'invention concerne un procédé permettant de déterminer la susceptibilité antibiotique d'un micro-organisme, selon les étapes suivantes: mélange de culture du micro-organisme dont la susceptibilité est étudiée avec un antibiotique par rapport auquel on souhaite tester la susceptibilité et un agent de perméabilisation du micro-organisme en quantité de perméabilisation efficace non inhibitrice de croissance, pour former une culture test; puis incubation de cette culture dans des conditions de culture appropriées et pendant une durée suffisante pour déterminer la susceptibilité du micro-organisme à l'antibiotique. Selon un autre aspect, l'invention concerne un procédé amélioré permettant de tester la susceptibilité antibiotique d'un micro-organisme dans une culture: on mélange la culture avec un antibiotique par rapport auquel la susceptibilité est testée, puis on procède à l'incubation de la culture pendant une durée suffisante pour déterminer la susceptibilité du micro-organisme à l'antibiotique; précisément, l'amélioration consiste à mélanger la culture avec un agent de perméabilisation du micro-organisme, en quantité de perméabilisation efficace non inhibitrice de croissance.

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