Device and method for dna amplification and assay

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) B01L 3/00 (2006.01) C12M 1/40 (2006.01) C12P 19/34 (2006.01) G01N 21/21 (2006.01) G01N 21/25 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2205057

A DNA amplification and homogeneous DNA probe assay device is provided which includes a multiplicity of discrete sample cells in a flat "card" format, with each sample cell containing the reagents necessary for both DNA amplification and homogeneous DNA probe assay. The device is particularly suitable for fluorescence polarization DNA probe assays, and is preferably provided with an integral polarizer to avoid the need for polarizing elements in the related measuring apparatus. The size and geometry of the sampe cells allows for a "hot start" of the DNA amplification reaction and thereby avoids mispriming of the amplification reaction.

L'invention se rapporte à un dispositif de dosage de sonde d'ADN homogène et d'amplification d'ADN comprenant une multiplicité de cellules d'échantillons distinctes, ce dispositif se présentant sous la forme d'une "carte" plate, chaque cellule d'échantillon contenant les réactifs nécessaires à l'amplification d'ADN et au dosage de la sonde d'ADN. Le dispositif est notamment approprié à des dosages de sonde d'ADN par polarisation en fluorescence, et est pourvu de préférence d'un polariseur solidaire évitant d'avoir recours aux éléments polarisants de l'appareil de mesure connexe. La taille et la géométrie des cellules d'échantillons permettent un "démarrage à chaud" de la réaction d'amplification de l'ADN et, par conséquent, empêchent un mauvais amorçage de la réaction d'amplification.

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