Device and method for multiple analyte detection

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/34 (2006.01) B01L 3/00 (2006.01) C12M 1/40 (2006.01) C12Q 1/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2250212

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample--distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead--end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

L'invention se rapporte à un procédé et à un dispositif permettant de tester simultanément un échantillon et déterminer la présence ou l'absence d'analyte et/ou la quantité d'au moins un analyte parmi une pluralité d'analytes sélectionnés. L'invention se rapporte, d'une part, à un dispositif de détection ou de quantification d'une pluralité d'analytes différents dans un échantillon liquide. Ce dispositif comprend un substrat qui forme un réseau de répartition des échantillons comportant (i) une admission, (ii) une ou plusieurs chambres de détection et (iii) des canaux formant un raccordement fluidique sans issue entre chacune des chambres et l'admission. Chaque chambre peut comporter un réactif spécifique d'un analyte, efficace pour réagir avec un analyte sélectionné éventuellement présent dans l'échantillon, et un élément de détection pour détecter le signal. L'invention se rapporte, d'autre part, à des procédés d'utilisation de ce dispositif.

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