Device and method for phage-based antibiotic susceptibility...

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B01L 3/00 (2006.01) B01L 3/14 (2006.01) B65D 81/32 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2196793

A phage-based antibiotic susceptibility test is carried out by maintaining a patient sample in a sealed well (12) during addition of the phage and Luciferin substrate used in the test, in order to prevent contamination of the laboratory environment. The phage is adhered in dried form to a metal carrier disk (26) which is retained beneath the cap (20) of the sealed sample well by means of an external magnet (24), and is mixed with the patient sample by removing the external magnet (24) and allowing the carrier disk (26) to fall to the bottom of the sample well (12). The Luciferin substrate is adhered to the underside of the cap (20) and is mixed with the patient sample by shaking or inverting the sealed sample well (12) after the metal carrier disk (26) has separated from the underside of the cap (20). A row of connected sample wells (12) and caps (20) may be employed to allow the same patient sample to be tested with multiple antibiotics.

La présente invention concerne la réalisation d'un antibiogramme à base de phage consistant à conserver, pendant l'adjonction du phage et du substrat de luciférine utilisé pour le test, le prélèvement du patient dans une éprouvette (12) étanche pour empêcher toute contamination de l'atmosphère du laboratoire. Le phage est collé sous forme sèche sur un disque métallique de support (26) maintenu grâce à un aimant extérieur (24) en dessous du bouchon (20) de l'éprouvette étanche, puis se mélange à l'échantillon prélevé sur le patient par simple retrait de l'aimant extérieur (24), le disque métallique de support (26) pouvant alors tomber au fond de l'éprouvette (12). Le substrat de luciférine étant collé contre la face inférieure du bouchon (20), une fois que le disque métallique de support (26) s'est séparé de la face inférieure du bouchon (20), il suffit de secouer ou de retourner l'éprouvette étanche (12) pour provoquer le mélange de l'échantillon prélevé sur le patient. En employant des éprouvettes (12) et des bouchons (20) montés en rangées, on a la possibilité de faire l'antibiogramme du même échantillon prélevé sur le patient avec différents antibiotiques.

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