Device and method for producing a replicate or derivative...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01)

Patent

CA 2756335

A method of producing a replicate or derivative of an array of molecules, the array comprising a spatial arrangement of separate samples of molecules, comprises creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the car-rier corresponds to the spatial arrangement of the samples in the array. The carrier comprising the copies of the samples is removed from the array.

Cette invention concerne une méthode permettant de produire une copie ou un dérivé d'un réseau de molécules, ledit réseau comprenant un agencement spatial d'échantillons de molécules distincts. Le procédé comprend les étapes consistant à créer, pour chaque échantillon, au moins une zone efficace limitée dans l'espace et distincte des zones efficaces des autres échantillons, l'aire, dotée d'un adaptateur de liaison ou de propriétés de liaison, d'un support bordant les zones efficaces. Les molécules sont amplifiées au moyen d'agents d'amplification dans les zones efficaces qui créent des copies ou des dérivés des échantillons. Les copies ou les dérivés des échantillons sont liés au support au moyen de l'adaptateur de liaison ou des propriétés de liaison, de sorte que l'agencement spatial des copies ou des dérivés des échantillons sur le support correspond à l'agencement spatial des échantillons dans le réseau. Le support comprenant les copies des échantillons est éliminé du réseau.

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