C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/08 (2006.01) A61K 35/18 (2006.01) A61M 1/36 (2006.01) C07K 14/705 (2006.01) C12M 1/26 (2006.01) C12M 1/34 (2006.01) C12N 5/06 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01) G01N 33/569 (2006.01) G02B 21/34 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2110292
2110292 9221746 PCTABS00017 The present invention provides in vitro models of the in vivo) rolling and arrest of leukocytes along the endothelial cell wall, which are important steps in the migration of leukocytes out of the blood stream and into tissue, as part of the inflammatory response. The in vitro models of the invention are functional under physiologic flow conditions resulting in physiologic shear stresses. In a specific embodiment, for modelling leukocyte rolling, the apparatus of the invention comprises a solid phase surface with rolling mediator molecules present thereon. Such rolling mediators are, for example, selectins and selectin ligands which have binding partners expressed on leukocytes. In another specific embodiment, for modelling leukocyte rolling followed by adhesion/arrest, the apparatus of the invention comprises a solid phase surface with both rolling mediators and integrin binding partners present thereon. The apparatuses of the invention can be used for collecting, concentrating, purifying, and analyzing blood and blood components, in particular, leukocytes and subsets thereof. The invention further relates to methods for identifying inhibitors or, alternatively, promoters (agonists, functional components) of the inflammatory response. Therapeutic and diagnostic methods, pharmaceutical compositions and kits are also provided.
Lawrence Michael
Springer Timothy A.
Center For Blood Research Inc.
Osler Hoskin & Harcourt Llp
LandOfFree
Device and method for the analysis of rolling blood... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Device and method for the analysis of rolling blood..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Device and method for the analysis of rolling blood... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1521524