Device and method for the quantitative determination of...

Details Device and method for the quantitative determination of... Device and method for the quantitative determination of...

C - Chemistry, Metallurgy

12

Q

150/11

C12Q 1/56 (2006.01)

Patent

CA 1197763

ABSTRACT OF THE DISCLOSURE Disclosed is a device for the quantitative determination of heparin in mammalian blood plasma, a method for the preparation of such device and a method for determining the plasma's heparin concen- tration using the device. The device, which is prepared using a freeze-drying technique, comprises a first upper layer of a carrier matrix material con- taining a buffer, AT-III and thrombin and a second, lower layer of a carrier matrix containing a thrombin sensitive fluorogenic or chromogenic substrate being capable of interacting with thrombin in such a manner that a time-related chemical change detectable by fluorometric or spectrophotometric means takes place when thrombin and the substrate are contacted in a suitable liquid environment.

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