Device and methods for measuring the response of at least...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/34 (2006.01)

Patent

CA 2457473

An apparatus and methods for measuring response of at least one cell to a medium, the response of at least one cell to a medium being characterized by a reaction time. In one embodiment of the present invention, the device (200) includes a membrane (206) and a side substrate (212) defining a sample well (269) in communication with the ambient air and for receiving the at least one cell (203) such that the membrane (206) is underneath the at least one cell. A sensor (205) is positioned underneath the second surface of the membrane (206), and an inlet (210) is in fluid communication with the sample well (269). A medium is introduced into the chamber through the inlet (210) to form a droplet that surrounds the cell, and the sensor measures the response of the cell to the medium at a time period shorter than the reaction time.

L'invention concerne un appareil et des procédés destinés à mesurer la réponse d'au moins une cellule face à un milieu. Cette réponse d'au moins une cellule face à un milieu étant caractérisée par un temps de réaction. Dans l'un des modes de réalisation de cette invention, le dispositif comprend une membrane et un substrat latéral définissant un puits à échantillons en communication avec l'air ambiant et permettant de recevoir cette/ces cellule(s) de sorte que la membrane se trouve au-dessous de cette/ces cellule(s). Un capteur est positionné au-dessous de la seconde surface de la membrane; et une entrée est en communication fluidique avec le puits à échantillons. Un milieu est introduit dans la chambre à travers l'entrée en vue de former une gouttelette qui entoure la cellule, et le capteur mesure la réponse de la cellule face au milieu pendant un temps donné plus court que le temps de réaction.

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