Device and process for cell capture and recovery

Details Device and process for cell capture and recovery Device and process for cell capture and recovery

C - Chemistry, Metallurgy

12

N

167/104, 167/45,

C12N 5/08 (2006.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01) C12N 5/06 (2006.01) C12N 7/02 (2006.01) G01N 33/554 (2006.01)

Patent

CA 1340565

Devices, processes and compositions are provided for effective separation of cells from a mixture of cells, where depletion or positive selection may be employed to provide a cellular population of interest. Of particular utility is the separation of cells from peripheral blood mononuclear cells, where members of the lymphoid or myeloid lineages may be isolated and used for research, diagnosis or therapy. Also of interest are cellular separation from bone marrow, tumor suspensions or lymphoid tissue suspensions, where cells can be isolated and used for a variety of purposes. The separated cells may be homogeneous, free of exogenous biologicals, viable, capable of replication and exhibit their full complement of biological activities. Multiple phenotypes can be captured simultaneously. Captured cells can be specifically activated with cytokines and antigens to provide cells which are MHC restricted and have antigen-specific effector functions.

613945

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