C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
204/182, 204/96,
C12N 13/00 (2006.01) C12M 3/00 (2006.01)
Patent
CA 1248045
ABSTRACT OF THE DISCLOSURE The present invention discloses a device and process for inducing membrane fusion under an electric field. The process comprises the steps of (a) suspending said membranes in an aqueous buffered medium in a manner so that the membranes are without contact with each other; (b) altering said membranes to a fusogenic state by applying 2-30 pulses of direct current at a rate of 2 to 5 pulses per second in an electrical field strength of about 500-1000 volts/mm in the medium,said pulses having a rapid rise time not exceeding about 10 micro seconds and an exponential decay half time of about 0.2 milli seconds to about 1.2 milliseconds; and (c) thereafter bringing said fusogenic membranes in contact with each other. A long-lived metastable fusogenic state of membranes has been possible to be obtained by the device and process of the present invention.
488251
American National Red Cross
Borden Ladner Gervais Llp
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