Device for cell culture

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/00 (2006.01) C12N 5/02 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2739771

The present invention relates to a device for cell culture, in particular of neuronal cells, comprising: a substrate defining a first microfluidic chamber to be seeded with a first cell culture, and at least a second mircofluidic chamber, a fluidic interconnection system connecting the first and second chambers and enabling cellular extensions, in particular axons, to extend from one chamber to the other, wherein the interconnection system of said device is made so as to promote the progression of at least one first type of cellular extension, said first and second types of extension being different either due to the microfluidic chamber from which they originate, or due to the type of cell of which they constitute an extension.

La présente invention concerne un dispositif de culture cellulaire, notamment de cellules neuronales, comportant : un support définissant une première chambre microfluidique destinée à être ensemencée, par une première culture cellulaire, et au moins une deuxième chambre microfluidique, un système d'interconnexion fluidique reliant les première et deuxième chambres et permettant à des extensions cellulaires, notamment dès axones, de s'étendre d'une chambre vers l'autre chambre, dispositif dans lequel le système d'interconnexion est réalisé de manière à privilégier la progression d'au moins un premier type d'extensions cellulaires par rapport à au moins un second type d'extensions cellulaires, lesdits premier et second types d'extensions cellulaires différant soit par la chambre microfluidique dont ils proviennent, soit par le type cellulaire dont ils sont l'extension.

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