C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/141, 167/327
C12N 5/08 (2006.01) A61F 2/06 (2006.01) A61L 27/00 (2006.01) C12M 3/00 (2006.01) C12M 3/08 (2006.01) C12N 3/00 (2006.01)
Patent
CA 2033927
DEVICE FOR COLLECTING AND PROCESSING FAT TISSUE TO PRODUCE ENDOTHELIAL CELL PRODUCT A B S T R A C T The invention is an endothelial cell procurement and deposition kit for collecting fat from a patient, processing said fat to produce an endothelial cell deposition product, and depositing said product on the surface of a graft, all under sterile conditions established and maintained within the components of said kit comprised of fat collection means for collecting subcutaneous fat from a patient; digestion means connectable to said fat collection means to maintain sterility during reception of said fat and for retaining said fat under sterile conditions during rinsing and digestion to produce a digested product; endothelial cell isolation means connectable to said digestion means for maintaining sterile conditions during reception of said digested product and for separating and isolating microvessel endothelial cells from said digested product to produce an endothelial cell product; cell deposition means connectable to said isolation means for maintaining sterile conditions during reception of said endothelial cell product and for depositing said cells on the surface of a graft to be implanted in a patient and facilitating implantation of said endothelial graft into a patient. A single device for collecting and processing tissue to produce endothelial cell product is provided.
Alchas Paul G.
Jarrell Bruce E.
Prais Alfred W.
Williams Stuart K.
Becton Dickinson And Company
Mitchell Richard J.
Thomas Jefferson University
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